The split method is simple and can be used in general families. It is suitable to select the spring and autumn rainy season to divide the plants. Use a sharp cutting tool to cut the root between the two "blue head" feet, carefully cut the root at the base, and disinfect the incision. Each clump needs at least 2 pseudobulbs with new buds.
In the early stage, liquid culture is mainly used to promote the rapid germination of seeds. After that, the protocorm can be directly inoculated on the medium to achieve one-step seedling formation. The specific steps are as follows:
When flowering, the healthy mother plant of Cattleya is selected for self pollination and hybridization. After pollination, the fruit will be basically mature in 120-150 days.
The mature fruit was soaked in 75% alcohol for half a minute, then sterilized. The fruit was put into 0.1% mercury solution, soaked for 20 minutes, washed with sterile water for 4 or 5 times, then cut the fruit, inoculated the white powder seed into the liquid medium for seed germination, and cultured in a shaking table at 150 rpm. One week later, the embryo germinated and formed green protocorm with diameter of 1-2 mm in 20 days.
Inoculate the protocorm with straw to the medium of seedling, one month can form a complete small plant, continue to cultivate for 45 days can grow into a strong big seedling.
When the plantlet can grow to 3,4 cm high, transfer to natural light for 1-2 weeks, and observe the formation of better cuticle. After taking it out of the glass bottle, wash the culture medium of the root, move it into the mixing pond cornerstone and carbonized bark matrix, keep the appropriate humidity, and do well in ventilation.
